張卉教授課題組在ANALYTICAL CHEMISTRY發(fā)表研究論文
Highly Sensitive Electrochemical Detection of Tumor Exosomes Based on Aptamer Recognition-Induced Multi-DNA Release and Cyclic Enzymatic Amplification
Dong, HL (Dong, Huilei)[ 1 ] ; Chen, HF (Chen, Hongfei)[ 1 ] ; Jiang, JQ (Jiang, Juqian)[ 1 ] ; Zhang, H (Zhang, Hui)[ 1 ]*（張卉）; Cai, CX (Cai, Chenxin)[ 1 ] ; Shen, QM (Shen, Qingming)[ 2,3 ]*
[ 1 ] Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Coll Chem & Mat Sci,Jiangsu Key Lab Biomed Mat, Natl & Local Joint Engn Res Ctr Biomed Funct Mat, Nanjing 210097, Jiangsu, Peoples R China
[ 2 ] Nanjing Univ Posts & Telecommun, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Key Lab Organ Elect & Informat Displays, Nanjing 210023, Jiangsu, Peoples R China
[ 3 ] Nanjing Univ Posts & Telecommun, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Inst Adv Mat, Nanjing 210023, Jiangsu, Peoples R China
ANALYTICAL CHEMISTRY，201804,90(7),4507-4513
Sensitive and specific detection of tumor exosomes is of great significance for early cancer diagnosis. In this paper, we report an aptamer strategy for exosome detection based on aptamer recognition induced multi-DNA release and cyclic enzymatic amplification. First, we use aptamer magnetic bead bioconjugates to capture tumor exosomes derived from LNCaP cells, leading to the release of three kinds of messenger DNAs (mDNAs). After magnetic separation, the released mDNAs hybridized with the probe DNAs immobilized on a gold electrode. Electroactive Ru(NH3)(3+)(6) was used as the signal reporter because of its electrostatic attraction to DNA. Subsequent Exo III cyclic digestion caused the electrochemical signal to "turn off'. Because the electrochemical signal reflects the concentration of Ru(NH3)(3+)(6) and the concentration of Ru(NH3)(3+)(6) is correlated with the mDNA concentration, which is correlated with the exosome concentration, the tumor exosomes can be detected by examining the decrease in the peak current of Ru(NH3)(3+)(6). In this paper, the signal was amplified by the numerous mDNAs released from the magnetic bead and the Exo III-assisted mDNA recycling. Under the optimal conditions, a detection limit down to 70 partides/mu L was achieved, which is lower than the LODs of most currently available methods. Furthermore, this assay can be used to detect tumor exosomes in complex biological samples, demonstrating potential application in real sample diagnosis.
文章鏈接：
https://pubs.acs.org/doi/10.1021/acs.analchem.7b04863
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