黃曉華教授課題組在JOURNAL OF INORGANIC BIOCHEMISTRY發(fā)表研究論文
Direct imaging of how lanthanides break the normal evolution of plants
Yang, Q (Yang, Qing)[ 1 ] ; Wang, LH (Wang, Lihong)[ 2 ] ; He, JF (He, Jingfang)[ 1 ] ; Yang, ZB (Yang, Zhenbiao)[ 3 ] ; Huang, XH (Huang, Xiaohua)[ 1 ]*（黃曉華）
[ 1 ] Nanjing Normal Univ, Sch Chem & Mat Sci, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Natl & Local Joint Engn Res Ctr Biomed Funct Mat, Nanjing 210046, Jiangsu, Peoples R China
[ 2 ] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
[ 3 ] Univ Calif Riverside, Ctr Plant Cell Biol, Inst Integrat Genome Biol, Riverside, CA 92521 USA
JOURNAL OF INORGANIC BIOCHEMISTRY，201805, 182，158-169
After rare earth elements [REE(III)] are anchored outside of the plasma membrane, REE(III) break plant evolution to initiate leaf cell endocytosis, which finally affects plant growth. However, the molecule for anchoring REE(III) in the acidic environment outside of the plasma membrane is not clear, which is crucial for exploring the mechanism of REE(III) breaking plant evolution. Here, lanthanum(III) [La(III)] and terbium(III) [Tb(III)] were respectively served as a representative of REE(III) without and with f electrons, and Arabidopsis was served as a representative of plants, cellular and molecular basis for arabinogalactan proteins (AGP) anchoring REE(III) outside of the plasma membrane was investigated. By using interdisciplinary methods, when REE(III) initiated leaf cell phagocytosis, we observed the increase in the expression of AGP and their migration to the outside of the plasma membrane. In the acidic environment outside of the plasma membrane, Tb(III) formed more stable Lewis acid-base [REE(III)-AGP] complexes with a higher apparent binding constant (1.51 x 10(-6)) than La(III) (1.24 x 10(-6)). In REE(III)-AGP complexes, the bond lengths of REE(III)-O were in normal range and H-bonds were strong H-bonds. The formation of REE(III)-AGP complexes sequentially disturbed the secondary and tertiary structure of AGP, which were enhanced with increasing the concentration of REE(III), and Tb(III) caused stronger structural changes than La(III). Hence, AGP could be molecules for anchoring REE(III) outside of the plasma membrane. The results of this study are direct imaging of how lanthanides break the normal evolution of plants, and can serve as an important guidance for investigating mechanism of lanthanides in organisms.
文章鏈接：
https://www.sciencedirect.com/science/article/pii/S0162013417307122?via%3Dihub
版權(quán)與免責(zé)聲明：本網(wǎng)頁的內(nèi)容由收集互聯(lián)網(wǎng)上公開發(fā)布的信息整理獲得。目的在于傳遞信息及分享，并不意味著贊同其觀點(diǎn)或證實(shí)其真實(shí)性，也不構(gòu)成其他建議。僅提供交流平臺(tái)，不為其版權(quán)負(fù)責(zé)。如涉及侵權(quán)，請(qǐng)聯(lián)系我們及時(shí)修改或刪除。郵箱：sales@allpeptide.com